Oil and grease wastewater were always treated with acidification and dissolved air flotation process. The concentrated greasy sludge should be separated and disposed to the sanitary landfill or combustion. This secondary oil treatment process attributed to high cost and the residual pollution. In this study, an innovative design of aerobic fluidized bed (AFB) bioreactor, was achieved with a pilot plant of one cubic meter volume and a down-flow jet aeration system. The volumetric loading rate of 2 kgCOD/m3-day and the oil inflow concentration of 600-1,000 mg/L associated with 2,000-3,000 mg/L of COD was applied to the first stage of AFB reactor. Supplement of diatomaceous earth carrier adsorption enhanced the oil attachment and biodegradation with partial oxidation. 0.19 to 0.22 kg oil/grease/m3-day was attained with good removal efficiency of 70 to 80% of oil and grease. The residual organic substrate was utilized by the acidogene associated with hydrogen production in the second state. Then the methanogene degrade the volatile fatty acids to produce methane in the third stage of anaerobic process. Two molecular biomonitorings of DGGE and T-RFLP methodologies were established for the ecological indicates of these three-phase microorganisms. With the long-term biomonitorings data base, the integrate bioprocess for oil and grease wastewater treatment could be controlled for the performance optimization. This wholesome biotechnology will be transferred to food industry as a Molecular Biomoniforing Process Technology (MBPT).

oil and grease,aerobic fermentation,oil and grease degrading bacteria